"In vitro reprogramming of fibroblasts into a pluripotent
ES-cell-like state," by Marius Wernig and 7 others,
Nature, 448(7151): 318-24, 19 July 2007.
Paste paper here. the source below is for all whats hot in papers
[Authors' affiliations: Whitehead Institute, MIT, Cambridge, MA;
Massachusetts General Hospital, Boston; Broad Institute, Boston; Harvard
Medical School, Boston]
Abstract: "Nuclear transplantation can reprogramme a
somatic genome back into an embryonic epigenetic state, and the
reprogrammed nucleus can create a cloned animal or produce pluripotent
embryonic stem cells. One potential use of the nuclear cloning approach is
the derivation of 'customized' embryonic stem (ES) cells for
patient-specific cell treatment, but technical and ethical considerations
impede the therapeutic application of this technology. Reprogramming of
fibroblasts to a pluripotent state can be induced in vitro through ectopic
expression of the four transcription factors Oct4 (also called Oct3/4 or
Pou5f1), Sox2, c-Myc and Klf4. Here we show that DNA methylation, gene
expression and chromatin state of such induced reprogrammed stem cells are
similar to those of ES cells. Notably, the cells--derived from mouse
fibroblasts--can form viable chimaeras, can contribute to the germ line and
can generate live late-term embryos when injected into tetraploid
blastocysts. Our results show that the biological potency and epigenetic
state of in-vitro-reprogrammed induced pluripotent stem cells are
indistinguishable from those of ES cells."
This 2007 report from Nature was cited 40
times in current journal articles indexed by Thomson Reuters
during March-April 2008. With this latest two-month total, the report
currently stands as the third-most-cited biology paper (aside from reviews)
published in the last two years. Prior to the most recent bimonthly count,
citations to the paper have accrued as follows:
January-February 2008: 40 citations
November-December 2007: 20
September-October 2007: 8
July-August 2007: 1
Total citations to date: 109
SOURCE:
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